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  • دکتری (1380)

    بیوتکنولوژی گیاهی

    UPM، کوالامپور،

  • کارشناسی‌ارشد (1371)

    اصلاح نباتات

    تهران، تهران، ایران

  • بیوتکنولوژی گیاهی
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  • نانوبیوتکنولوژی کشاورزی و محیط زیست
  • ایمنی زیستی و قوانین و مقررات مربوطه

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    رزومه

    Improving productivity of steviol glycosides in Stevia rebaudiana via induced polyploidy

    Daryush Talei, Mojtaba Khayam Nekouei, Mohsen Mardi, Saeid Kadkhodaei
    Journal PapersJournal of Crop Science and Biotechnology , Volume 23 , Issue 4, 2020 September , {Pages 301-309 }

    Abstract

    The polyploidy induction using mutagenic chemicals is one of the breeding approaches to improve enhancing productivity of yield and secondary metabolites in medicinal plants. In the present research, to induce polyploidy in Stevia rebaudiana plants, the seeds were treated with four concentrations of colchicine (control, 0.05%, 0.1% and 0.2%) for 12, 24, 36 and 48?h in a factorial experiment based on randomized complete block design with three replicates. The morphological and phytochemical traits of plants were measured before the flowering stage. The results indicated that different concentrations of colchicine had a significant effect on the plant height, number of leaves, number of branches, mean of leaf length, stomata size and stomata

    Improving productivity of steviol glycosides in Stevia rebaudiana via induced polyploidy

    Saeid Kadkhodaei, Mojtaba Khayam Nekouei, Daryush Talei, Mohsen Mardi
    Journal PapersJournal of Crop Science and Biotechnology , Volume 2020 , Issue 2020, 2020 April 15, {Pages }

    Abstract

    The polyploidy induction using mutagenic chemicals is one of the breeding approaches to improve enhancing productivity of yield and secondary metabolites in medicinal plants. In the present research, to induce polyploidy in Stevia rebaudiana plants, the seeds were treated with four concentrations of colchicine (control, 0.05, 0.1 and 0.2) for 12, 24, 36 and 48 h in a factorial experiment based on randomized complete block design with three replicates. The morphological and phytochemical traits of plants were measured before the flowering stage. The results indicated that different concentrations of colchicine had a significant effect on the plant height, number of leaves, number of branches, mean of leaf length, stomata size and stomata den

    Evaluation of freezing tolerance in four olive cultivars (native and foreign) based on physiological indicators under cold acclimation conditions

    T Basaki, M Khayam Nekouei, S Karami, S Faraji, M Jalili
    Journal Papers , , {Pages }

    Abstract

    Evaluation of Iranian pomegranate collection using simple sequence repeat and morphological traits

    T Basaki, M Khayam Nekouei, R Choukan, M Mardi
    Journal PapersCrop Breeding Journal , Volume 2 , 2016 January , {Pages 67-78 }

    Abstract

    Pomegranate, Punica granatum L., is one of the oldest cultivated fruit species. This study used morphological data and a set of simple sequence repeat markers to investigategenetic diversity among 202 Iranian pomegranate accessions during the 2010 and 2011 growing seasons at Saveh Research Station, Saveh, Iran. Principal component analysis showed that leaf traits were predominant in the first and second component during both years, indicating that these traits are not only useful in assessing genetic diversity, but also for characterizing pomegranate germplasm. There was high correlation between the length of style and flower shape, implying that these traits are directly associated with tree performance. There was also close correlation be

    Development of a bioprocess for fast production of enriched biocompost from municipal solid wastes

    Helen Pourmazaheri, Gholamreza Salehi Jouzani, Ebrahim Karimi, Seyed Mojtaba Khayam Nekouei, Meisam Tabatabaei, Reza Maali Amiri
    Journal PapersInternational Biodeterioration & Biodegradation , Volume 104 , 2015 October 1, {Pages 482-489 }

    Abstract

    The objective of the present study was to develop a bioprocess for fast production of enriched biocompost from municipal solid wastes using a native microbial cocktail and cheap lignocellulosic biomass i.e. wood chips. The open-windrow composting experiments included (1) only municipal solid waste (C), (2) municipal solid waste?+?wood chips at 3:1 ratio (CW), and (3) municipal solid waste?+?wood chips?+?a microbial cocktail (108?cells/kg), containing 11 native mesophilic and thermophilic bacterial strains (CWM).The microbial cocktail led to the fastest rise in the starting temperature (up to 73??C after two weeks) and maximum carbon/nitrogen ratio decrease (40%) and organic matter reduction in the CWM compost. The CWM compost contained the

    Identification of Natural Nucleotide Mutations of Salt Tolerance Candidate Genes (HvHKT1 and HvCBL4) in Barley Ecotypes by Sequencing

    Adnan Al-Yassin, Raheleh Khademian, Mohsen Mardi, Seyed Mojtaba Khayam Nekouei, Babak Nakhoda
    Journal Papers , 2015 January , {Pages }

    Abstract

    This study was conducted to identify single nucleotide variation in salinity stress tolerance candidate genes of barley ecotypes and to search for new diversity resources. Therefore, 96 barley ecotypes, collected from different areas worldwide, were used and two salinity tolerance candidate genes (HvHKT1 and HvCBL4) were targeted through sequencing. Genomic DNA fragments of the ecotypes were amplified by eight pairs of primers of the genes (4 pairs for each gene) by PCR reaction. Total number of nucleotide variation in HvHKT1 was significantly higher than in HvCBL4. Results of comparing sequence of these two genes showed that 17.9 SNP/Kb, 1 indel per 199.3 bp for HvHKT1 and 137SNP/kb, 18 indel per bp for HvCBL4. SNP frequency was lower than

    Application of Microsatellite Markers for Identification and Registration of Pistachio Cultivars

    M Mardi, M Zeynal Abedini, A Taj Abadi
    Journal Papers , , {Pages }

    Abstract

    Assessment of genetic diversity and genetic relationships among 46 Iranian and non-Iranian dwarfing rootstocks of apple (Malus? domestica Borkh.) using microsat...

    P Fakhrian, M Zeinalabedini, SM Khayam-Nekouei, S Kianamiri, SM Pirseyedi
    Journal PapersThe Journal of Horticultural Science and Biotechnology , Volume 89 , Issue 2, 2014 January 1, {Pages 121-129 }

    Abstract

    Dwarfing apple rootstocks are essential in modern apple production for the control of vigour, increased disease resistance, precocity, and predictability in orchard management. Such rootstocks also decrease the cost of production by lowering the costs of pruning, spraying, and harvesting. In this study, 18 new microsatellite (simple sequence repeat; SSR) markers were isolated and identified from a repeat sequence-enriched genomic library of Iranian dwarfing apple rootstocks using a modified Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO) procedure. GA/GT and AG repeat motifs were the most abundant di-nucleotides isolated. Nineteen microsatellite markers from previous work were also chosen for analysis in this study. Thirty-s

    Assessment of genetic diversity and genetic relationships among 46 Iranian and non-Iranian dwarfing rootstocks of apple (Malus? domestica Borkh.) using microsatellite markers

    P Fakhrian, M Zeinalabedini, SM Khayam-Nekouei, S Kianamiri, SM Pirseyedi
    Journal PapersThe Journal of Horticultural Science and Biotechnology , Volume 89 , Issue 2, 2014 January 1, {Pages 121-129 }

    Abstract

    Dwarfing apple rootstocks are essential in modern apple production for the control of vigour, increased disease resistance, precocity, and predictability in orchard management. Such rootstocks also decrease the cost of production by lowering the costs of pruning, spraying, and harvesting. In this study, 18 new microsatellite (simple sequence repeat; SSR) markers were isolated and identified from a repeat sequence-enriched genomic library of Iranian dwarfing apple rootstocks using a modified Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO) procedure. GA/GT and AG repeat motifs were the most abundant di-nucleotides isolated. Nineteen microsatellite markers from previous work were also chosen for analysis in this study. Thirty-s

    Taxonomic study of a salt tolerant Streptomyces sp. strain C-2012 and the effect of salt and ectoine on lon expression level

    Akram Sadeghi, Bahram M Soltani, Gholamreza Salehi Jouzani, Ebrahim Karimi, Mojtaba Khayam Nekouei, Majid Sadeghizadeh
    Journal PapersMicrobiological research , Volume 169 , Issue 03-Feb, 2014 February 1, {Pages 232-238 }

    Abstract

    Streptomyces strain C-2012 is a salt tolerant biocontrol PGPR that has been isolated from Iranian soil. The main aim of current study was finding strain C-2012 taxonomic position and to find the genes which are potentially involved in salt tolerance phenotype. Strain C-2012 chemotaxonomic, morphological and molecular characteristics indicate that this strain is a member of the genus Streptomyces. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence revealed that this strain is closely related to Streptomyces rimosus JCM 4667T. Also, DNA–DNA hybridization test estimated 74% relatedness between two strains and confirmed that C-2012 is a strain of S. rimosus. In order to find novel genes that are differentially expressed

    Diversity of the ectoines biosynthesis genes in the salt tolerant Streptomyces and evidence for inductive effect of ectoines on their accumulation

    Akram Sadeghi, Bahram M Soltani, Mojtaba Khayam Nekouei, Gholamreza Salehi Jouzani, Hossein Hadavand Mirzaei, Majid Sadeghizadeh
    Journal PapersMicrobiological research , Volume 169 , Issue 10-Sep, 2014 September 1, {Pages 699-708 }

    Abstract

    Streptomyces commonly produce ectoines as compatible solutes to prevent osmotic stresses. Fine structure of the genes producing ectoine (ectC) and hydroxyectoine (ectD) enzymes in Streptomyces rimosus C-2012 as a slightly halophilic bacterium is reported in this study. Deduced amino acid sequences of ectC and ectD genes from strain C-2012 and some other related species were compared and 72–90% and 13–81% identities were detected for ectC and ectD, respectively. High similarity of ectC between closely or distantly related Streptomyces to the strain C-2012 may indicate horizontal transfer of this gene. However, phylogenetic relationships of ectD were correlated with phylogenetic affiliation of the strains. It suggests that the ability of

    Development of 12 new SSR markers for genetic diversity and structure analysis in pistachio (Pistacia vera L.)

    SH Kolahi-Zonoozi, M Mardi, M Zeinalabedini, SM Pirseyedi, P Mahmoodi, I Tabatabaei, SM Mosavi-Derazmahalleh, M Farsi, MA Ebrahimi, K Ahmadi
    Journal PapersThe Journal of Horticultural Science and Biotechnology , Volume 89 , Issue 6, 2014 January 1, {Pages 707-711 }

    Abstract

    Pistachio (Pistacia vera L.) is an important horticultural crop with valuable nuts. So far, only a limited number of microsatellite (simple sequence repeat; SSR) markers have been developed for members of the genus Pistacia, among which, only a few have shown polymorphism among genotypes of Iranian pistachio. This limits precise genotyping and diversity studies on this commercially valuable species. In the present study, we report the development of 12 novel microsatellite (SSR) markers isolated from a repeat sequence-enriched genomic library of P. vera L. We assessed genetic structure and diversity among 45 commercially important Iranian cultivars using these 12 markers. Polymorphic information contents (PIC) varied from 0.19 – 0.56, wit

    Assessment of genetic diversity and genetic relationships among 46 Iranian and non-Iranian dwarfing rootstocks of apple (Malus× domestica Borkh.) using microsatellite markers

    P Fakhrian, M Zeinalabedini, SM Khayam-Nekouei, S Kianamiri, ...
    Journal Papers , , {Pages }

    Abstract

    EFFECT OF DIFFERENT GROWTH REGULATORS LEVELS AND EXPLANTS TYPES OF CALLOGENESIS AND ORGANOGENESISHYPERICUM PERFORATUM L. UNDER IN VITRO CONDITION

    E SHARAFI, NEKOUEI SM KHAYAM, MH FOTOKIAN, D DAVOODI, T HASSANLOO
    Journal Papers , Volume 5 , Issue 3, 2013 January 1, {Pages 57-66 }

    Abstract

    In this study the effect of different levels of plant growth regulators (picloram, 2, 4-D, IAA and BAP) and explants type (root, stem and leaf) were examined on callogenesis and organogenesis of st John's wort with factorial experiment design on the basis of completely randomized design with five repeats and five explants. The explants were obtained from 30 days plantlets and transferred to media supplemented with picloram, 2, 4-D, IAA (0, 0.5, 1, 2 mgl-1) and BAP (0, 0.4, 0.8 mg l-1). Samples were kept in the growth chamber in the darkness condition at 23 o C. After 28 days, the interactions between explants types and hormone levels were investigated. The significant effect at the 1 percent probability was observed between treatment levels

    A proteomics approach to study the molecular basis of enhanced salt tolerance in barley (Hordeum vulgare L.) conferred by the root mutualistic fungus Piriformospor...

    Mehdi Alikhani, Behnam Khatabi, Mozhgan Sepehri, Mojtaba Khayam Nekouei, Mohsen Mardi, Ghasem Hosseini Salekdeh
    Journal PapersMolecular BioSystems , Volume 9 , Issue 6, 2013 January , {Pages 1498-1510 }

    Abstract

    Piriformospora indica is a root-interacting mutualistic fungus capable of enhancing plant growth, increasing plant resistance to a wide variety of pathogens, and improving plant stress tolerance under extreme environmental conditions. Understanding the molecular mechanisms by which P. indica can improve plant tolerance to stresses will pave the way to identifying the major mechanisms underlying plant adaptability to environmental stresses. We conducted greenhouse experiments at three different salt levels (0, 100 and 300 mM NaCl) on barley (Hordeum vulgare L.) cultivar “Pallas” inoculated with P. indica. Based on the analysis of variance, P. indica had a significant impact on the barley growth and shoot biomass under normal and salt str

    EFFECT OF DIFFERENT GROWTH REGULATORS LEVELS AND EXPLANTS TYPES OF CALLOGENESIS AND ORGANOGENESISHYPERICUM PERFORATUM L....

    E SHARAFI, NEKOUEI SM KHAYAM, MH FOTOKIAN, D DAVOODI, T HASSANLOO
    Journal Papers , Volume 5 , Issue 3, 2013 January 1, {Pages 57-66 }

    Abstract

    In this study the effect of different levels of plant growth regulators (picloram, 2, 4-D, IAA and BAP) and explants type (root, stem and leaf) were examined on callogenesis and organogenesis of st John's wort with factorial experiment design on the basis of completely randomized design with five repeats and five explants. The explants were obtained from 30 days plantlets and transferred to media supplemented with picloram, 2, 4-D, IAA (0, 0.5, 1, 2 mgl-1) and BAP (0, 0.4, 0.8 mg l-1). Samples were kept in the growth chamber in the darkness condition at 23 o C. After 28 days, the interactions between explants types and hormone levels were investigated. The significant effect at the 1 percent probability was observed between treatment levels

    Shotgun Proteomic Analysis of the Mexican Lime Tree Infected with “CandidatusPhytoplasma aurantifolia”

    Aboozar Monavarfeshani, Mehdi Mirzaei, Elham Sarhadi, Ardeshir Amirkhani, Mojtaba Khayam Nekouei, Paul A Haynes, Mohsen Mardi, Ghasem Hosseini Salekdeh
    Journal PapersJournal of proteome research , Volume 12 , Issue 2, 2013 January 7, {Pages 785-795 }

    Abstract

    Infection of Mexican lime trees (Citrus aurantifolia L.) with the specialized bacterium “CandidatusPhytoplasma aurantifolia” causes witches’ broom disease. Witches’ broom disease has the potential to cause significant economic losses throughout western Asia and North Africa. We used label-free quantitative shotgun proteomics to study changes in the proteome of Mexican lime trees in response to infection by “Ca. Phytoplasma aurantifolia”. Of 990 proteins present in five replicates of healthy and infected plants, the abundances of 448 proteins changed significantly in response to phytoplasma infection. Of these, 274 proteins were less abundant in infected plants than in healthy plants, and 174 proteins were more abundant in infect

    Improvement of silymarin production in hairy root cultures of Silybum marianum (L.) Gaertn using fungal elicitors

    Tahereh Hasanloo, MASUMEH Ahmadi, SM Khayyam Nekoei, GH Salehi Jozani
    Journal PapersRom Biotechnol Lett , Volume 18 , Issue 3, 2013 May 1, {Pages }

    Abstract

    The objective of the present study was to enhance silymarin production in hairy root cultures of Silybum marianum (L.) Gaertn using fungal elicitors. The effects of different concentrations of the fungal elicitors (0, 10 and 20 mg/50 ml culture), including Fusarium proliferatum, Aspergillus niger, and Rhizoctonia solani, were studied on silymarin production in the S. marianum hairy root cultures. The hairy roots were harvested 0, 24, 48 and 72 h after inoculations. Detection and identification of flavonolignans was carried out by high performance liquid chromatography method. The maximum silymarin production for each of the fungi treatments were as follow: 10 mg A. niger/50 ml culture (0.18 mg/g DW) after 48 h, 20 mg/50 ml culture F. prolif

    Phytoplasma-responsive microRNAs modulate hormonal, nutritional, and stress signalling pathways in Mexican lime trees

    Farveh Ehya, Aboozar Monavarfeshani, Ehsan Mohseni Fard, Laleh Karimi Farsad, Mojtaba Khayam Nekouei, Mohsen Mardi, Ghasem Hosseini Salekdeh
    Journal PapersPloS one , Volume 8 , Issue 6, 2013 June 18, {Pages e66372 }

    Abstract

    Background Witches’ broom disease of Mexican lime (Citrus aurantifolia L.), which is associated to the phytoplasma ‘Candidatus Phytoplasma aurantifolia’, is a devastating disease that results in significant economic losses. Plants adapt to biotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) are a recently identified family of molecules that regulate plant responses to environmental stresses through post-transcriptional gene silencing. Methods Using a high-throughput approach to sequence small RNAs, we compared the expression profiles of miRNAs in healthy Mexican lime trees and in plants infected with ‘Ca. P. aurantifolia’. Results Our results demonstrated the

    Micropropagation of tuna (Opuntia ficus–indica) and effect of medium composition on proliferation and rooting

    Akram Ghaffari, Tahereh Hasanloo, Mojtaba Khayam Nekouei
    Journal PapersInt J Biosci , Volume 3 , 2013 January , {Pages 129-139 }

    Abstract

    The goal of this study was to determine micropropagation system for a mass production of Tuna (Opuntia ficus–indica). For this reason, explants dissected from strilled young cladodes successfully established on Murashige and Skoog (MS) medium supplemented with 5 mg l-1 Benzyl amino purine (BAP). MS medium containing different combinations of BAP (5 mg l-1) and Indole acetic acid (IAA)(0, 0.25, 0.5, 1, 2 mg l-1), BAP (5 mg l-1) and Naphtalene acetic acid (NAA)(0, 0.25, 0.5, 1, 2 mg l-1) and BAP (0.5 and 1 mg l-1) and Kinetin (0.5 and 1 mg l-1) were tested for shoot development. The best results for shoot development and elongation were obtained in media containing 5 mg l-1 and 0.25 mg l-1 NAA. The highest multiplication rate (3.9) was obse

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    دروس نیمسال جاری

    • كارشناسي ارشد
      زيست فناوري ( واحد)
      دانشکده علوم زیستی، گروه بيوشيمي

    دروس نیمسال قبل

    • كارشناسي ارشد
      اصول پژوهش و فناوري ( واحد)
      دانشکده علوم زیستی، گروه بيوشيمي
    • كارشناسي ارشد
      كاربردهاي ريز زيست فناوري ( واحد)
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